Transposon

Transposons are sequences of DNA that can move around to different positions within the genome of a single cell as a block of conserved DNA sequence, a process called transposition. In the process, they can cause mutations and when they duplicate in this process can change the amount of DNA in the genome. Transposons are also called "jumping genes", mobile DNA or "mobile genetic elements". Discovered by Barbara McClintock early in her career, the topic went on to be a Nobel winning work in 1983.

Transposons insert at new locations in DNA by enzyme catalysed breakage and reunion of DNA, which is called DNA recombination. The distinctive feature of all transposon movement is that there is no requirement for extensive DNA sequence similarity (that is to say, extensive complementary base pairing) between the intial DNA region containing the transposon and the final location. Thus the process of transposon insertion at a new location is classified as nonhomologous recombination to distinguish it from DNA breakage and reunion processes affecting similar DNA sequences during homologous recombination.

There are a variety of mobile genetic elements, and they can be grouped based on their mechanism of transposition. Class I mobile genetic elements, or retrotransposons, move in the genome by being transcribed to RNA and then back to DNA by reverse transcriptase, while class II mobile genetic elements move directly from one position to another within the genome using a transposase enzyme catalyst to mobilize the DNA without converting it to an RNA intermediate.

Transposons are very useful to researchers as a means to alter DNA inside of a living organism.

Transposons make up a large fraction of genome sizes which is evident through the C-values of eukaryotic species. As an example about 45% of the human genome is composed of transposons and their defunct remnants.

Types of transposons

Transposons are classified into three main classes based on their mechanism of transposition.

Class I: Retrotransposons

Retrotransposons work by copying themselves and pasting copies back into the genome in multiple places. Initially retrotransposons copy themselves to RNA (transcription) but, in addition to being translated, the RNA is copied into DNA by a reverse transcriptase (often coded by the transposon itself) and inserted back into the genome.

Retrotransposons behave very similarly to retroviruses, such as HIV, giving a clue to the evolutionary origins of such viruses. Retrotransposons are common in eukaryotic organsim 9for instance maize, humans), but are rarely found in bacteria. They are present in fungi.

There are three main classes of Retrotransposons:

• Viral superfamily: similar to retroviruses, have long terminal repeats (LTRs), encode reverse transcriptase (to reverse transcribe RNA into DNA).
• LINES: encode reverse transcriptase (to reverse transcribe RNA into DNA), lack LTRs, transcribed by RNA polymerase II.
• Nonviral superfamily: do not code for reverse transcriptase, transcribed by RNA polymerase III

Class II:DNA Transposons

The major difference of Class II transposons from retrotransposons is that their transposition mechanism does not involve an RNA intermediate. Class II transposons usually move by cut and paste, rather than copy and paste, using the transposase enzyme. Different types of transposase work in different ways. Some can bind to any part of the DNA molecule, and the target site can therefore be anywhere, while others bind to specific sequences. Transposase makes a staggered cut at the target site producing sticky ends, cuts out the transposon and ligates it into the target site. A DNA polymerase fills in the resulting gaps from the sticky ends and DNA ligase closes the sugar-phosphate backbone. This results in target site duplication and the insertion sites of DNA transposons may be identified by short direct repeats (a staggered cut in the target DNA filled by DNA polymerase) which flank the inverted repeats that are part of the transposon itself (and which are important for the transposon excision by transposase).

Not all DNA transposons transpose through cut and paste mechanism. In some cases a replicative transposition is observed in which transposon replicates itself to a new target site.

Both class I and class II of transposons may lose their ability to synthesise reverse transcriptase or transposase through mutation, yet continue to jump through the genome because other transposons are still producing the necessary enzyme. Transposons that themselves encode an ability to move to new locations are called autononomous transposons.

Class III: Miniature Inverted-repeat transposable elements

MITEs are sequences of about 400 base pairs and 15 base pair inverted repeats that vary very little. They are found in their thousands in the genomes of both plants and animals (over 100,000 were found in the rice genome). MITEs are too small to encode any proteins.

Examples

• The first transposons were discovered in maize (Zea mays), (aka corn) by Barbara McClintock in the 1940s, for which she was awarded a Nobel Prize in 1983. She noticed the results of insertions, deletions, and translocations, caused by these transposons. These changes in the genome could, for example, lead to a change in the color of corn kernels. About 50% of the total genome of maize consists of transposons. The Ac/Ds system McClintock described are class II transposons .
• One family of transposons in the fruit fly Drosophila melanogaster are called P elements. They seem to have first appeared in the species only in the middle of the twentieth century. Within 50 years, they have spread through every population of the species. Artificial P elements can be used to insert genes into Drosophila by injecting the embryo. For the use of P elements as a genetic tool see: "transposons as a genetic tool".
• The simplest form of bacterial transposon are insertion sequences (IS) which are about 1000 base pairs long and consist of inverted DNA repeats flanking a transposase gene. Other transposons in bacteria usually carry an additional gene for function other than transposition---often for antibiotic resistance. They can consist of two IS elements flanking a gene for antibiotic resistance

• In bacteria, transposons can jump from chromosomal DNA to plasmid DNA and back, allowing for the transfer and permanent addition of genes such as those encoding antibiotic resistance (multi-antibiotic resistant bacterial strains can be generated in this way). Bacterial transposons of this type belong to the Tn family. When the transposable elements lack additional genes, they are known as insertion sequences Snyder, L. and Champness, W. (2003)Transposion and site-specific recombination, Chapter 9 In Snyder, L. and Champness, W. Molecular Genetics of Bacteria. 2nd. Edition. ASM Press, Washington, DC. IS elements were first discovered in the gal gene of Escheria coli by James Shapiro in 1968.
• The most common form of transposon in humans is the Alu sequence. The Alu sequence is approximately 300 bases long and can be found between 300,000 and a million times in the human genome.
• Mu phage transposition is the best known example of replicative transposition. Its transposition mechanism is somewhat similar to a homologous recombination.

Transposons causing diseases

Transposons are mutagens. They can damage the genome of their host cell in different ways:

• A transposon or a retroposon that inserts itself into a functional gene will most likely disable that gene.
• After a transposon leaves a gene, the resulting gap will probably not be repaired correctly.
• Multiple copies of the same sequence, such as Alu sequences can hinder precise chromosomal pairing during mitosis, resulting in unequal crossovers, one of the main reasons for chromosome duplication.

Diseases that are often caused by transposons include hemophilia A and B, severe combined immunodeficiency, porphyria, predisposition to cancer, and Duchenne muscular dystrophy.

Additionally, many transposons contain promoters which drive transcription of their own transposase. These promoters can cause aberrant expression of linked genes, causing disease or mutant phenotypes.

Evolution of transposons

The evolution of transposons and their effect on genome evolution is currently a dynamic field of study.

Transposons are found in all major branches of life. They may or may not have originated in the last universal common ancestor, or arisen independently multiple times, or perhaps arisen once and then spread to other kingdoms by horizontal gene transfer. While transposons may confer some benefits on their hosts, they are generally considered to be selfish DNA parasites that live within the genome of cellular organisms. In this way, they are similar to viruses. Viruses and transposons also share features in their genome structure and biochemical abilities, leading to speculation that they share a common ancestor.

Since excessive transposon activity can destroy a genome, many organisms seem to have developed mechanisms to reduce transposition to a manageable level. Bacteria may undergo high rates of gene deletion as part of a mechanism to remove transposons and viruses from their genomes while eukaryotic organisms may have developed the RNA interference (RNAi) mechanism as a way of reducing transposon activity. In the nematode Caenorhabditis elegans, some genes required for RNAi also reduce transposon activity.

Transposons may have been co-opted by the vertebrate immune system as a means of producing antibody diversity. The V(D)J recombination system operates by a mechanism similar to that of transposons.

Evidence exists that transposable elements may act as mutators in bacteria and other asexual organisms.

Transposons in science

Transposons were first discovered in the plant maize. Likewise, the first transposon to be molecularly isolated was from a plant (Snapdragon). Appropriately, transposons have been an especially useful tool in plant molecular biology. Researchers use transposons as a means of mutagenesis. In this context, a transposon jumps into a gene and produces a mutation. The presence of the transposon provides a straightforward means of identifying the mutant allele, relative to chemical mutagenesis methods.

Sometimes the insertion of a transposon into a gene can disrupt that gene's function in a reversible manner; transposase mediated excision of the transposon restores gene function. This produces plants in which neighboring cells have different genotypes. This feature allows researchers to distinguish between genes that must be present inside of a cell in order to function (cell-autonomous) and genes that produce observable effects in cells other than those where the gene is expressed.

Transposons are also a widely used tool for mutagenesis in Drosophila melanogaster, and a wide variety of bacteria to study gene function. See: transposons as a genetic tool.

See also

• Mobile DNA
• Insertion sequence
• Intragenomic conflict

Further reading

• Bennetzen, J. L., 2000 Transposable element contributions to plant gene and genome evolution. Plant Molecular Biology 42: 251–269, 2000.
• Kidwell, M.G. (2005). Transposable elements. In The Evolution of the Genome (ed. T.R. Gregory), pp. 165-221. Elsevier, San Diego.
• Craig NL, Craigie R, Gellert M, and Lambowitz AM (ed.) (2002) Mobile DNA II, ASM Press, Washington, DC.
• Lewin B (2000) Genes VII, Oxford University Press.
• Snyder, L. and Champness, W. (2003) Transpositon and site-specific recombination, Chapter 9 In Snyder, L. and Champness, W. Molecular Genetics of Bacteria. 2nd. Edition. ASM Press, Washington, DC

External links

• Mechanism of Transposition (Animation)
• Kimball's Biology Pages: Transposons
• A possible connection between aberrant reinsertions and lymphoma - New Scientist