Deuterium: Difference between revisions
imported>Trevor Ebbens m (Extra neutron rather than proton.) |
imported>Paul Wormer m (→Use in NMR spectroscopy: typo) |
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== Use in NMR spectroscopy == | == Use in NMR spectroscopy == | ||
NMR spectroscopy, which primarily detects the signals of proton atoms, frequently uses a | NMR spectroscopy, which primarily detects the signals of proton atoms, frequently uses a deuterated solvent and/or a deuterated compound as a chemical shift reference. Deuterium atoms also have different relaxation properties so that their use in NMR spectroscopy of large [[biopolymers]] such as [[protein]]s leads to enhanced signal detection. | ||
===Deuterated NMR solvents=== | ===Deuterated NMR solvents=== | ||
Deuterated chemicals that are often used for NMR spectroscopy include: | Deuterated chemicals that are often used for NMR spectroscopy include: | ||
Revision as of 10:01, 23 March 2008
Deuterium, chemical symbol D or 2H is an isotope of the element hydrogen which has a nucleus containing one proton and one neutron. A compound containing deuterium is said to be deuterated. Deuterated chemicals are widely use in NMR spectroscopy, either as chemical shift references, as solvents, or to reduce the relaxation rates of NMR signals in large compounds like proteins. Deuterium is a stable, naturally occuring isotope of hydrogen and represents 0.015% of naturally occuring hydrogen, with H-1 representing the remaining 99.985%.
Use in NMR spectroscopy
NMR spectroscopy, which primarily detects the signals of proton atoms, frequently uses a deuterated solvent and/or a deuterated compound as a chemical shift reference. Deuterium atoms also have different relaxation properties so that their use in NMR spectroscopy of large biopolymers such as proteins leads to enhanced signal detection.
Deuterated NMR solvents
Deuterated chemicals that are often used for NMR spectroscopy include:
- d6-acetone (1,1,1,3,3,3-hexadeuteroacetone)
- d3-chloroform (1,1,1-trideutero-chloroform, trideuterochloromethane)
- d-ethanol
- d6-benzene (1,2,3,4,5,6-hexdeuterobenzene)
- d2-methylenechloride
Each of these chemicals have a small proportion of undeuterated content, which give a residual signal that can be used as a (non-zero) reference chemical shift. However, several chemical compounds are specifically used a true reference points.
Chemical shift references
Three deuterated chemicals are widely used reference points for 1H NMR data. In organic solvents, deutero- trimethylsilane (d9-TMS) is used. For aqueous solutions, deuterated DSS or TSP are used instead.